Conducting a crosstalk test using the leica tcs sp5 confocal microscope. All rights to this documentation are held by leica microsystems cms. The leica tcs sp5 mp covers a wide range of imaging applications multiphoton and one photon by combining two technologies in one system. Leica dm2500 upright fluorescent microscope manuals cell. The system provides the full range of scan speeds at the highest resolution with its proven, highefficiency sp detection five channels simultaneously and optional aobs acousto optical bream splitter, the leica tcs sp5 delivers bright, noisefree. Sp8 lightning confocal microscope products leica microsystems. The main focus of this operating manual is directed to the safety notes that must be strictly adhered to when working with the leica tcs sp5 and leica tcs sp5 x. Leica tcs sp5 confocal this is a very flexible and fast confocal system build with a leica dmi 6000 cs inverted, fully automated microscope with motorized stage, condenser, objective and filter turrets. Software wizards for frap, flip and fret 2 the system accomplishes the most demanding requirements in recent multifluorescence research by groundbreaking new technologies to maximize spectral and multichannel performance. The leica tcs sp5 is supplied with the latest version of the licensed las af software. User guide to the ibif leica tcs sp8 mp confocal microscope. Two of these detectors are very sensitive hyd sp gaaspdetectors.
A message will appear asking whether you want to initialize the stage. Conducting a zstack scan using the leica tcs sp5 confocal microscope. Leica tcs sp5 iibrochure en pdf, 3 mb leica tcs sp5 mpflyer. This manual is the first section of a three part leica tcs sp5 user guide. Leica sp5 training manual september 2010 for the argon laser, adjust.
Do you struggle with a variety of instruments, protocols, and software interfaces in your lab. We intend this book for the novice and the experienced user. Sp5 imaging and microscopy git nov05 pdf, 1 mb white confocal formatex 2007 pdf. Leica tcs sp5 confocal louisiana state university health. Instead, you are referred to the online help of the leica application suite. Software license the software described in this document is furnished under a license agreement which is included with the product. There is a standard, manual xy stage with slide holder, but this can be. Tcs sp5 ii leica tcs sp5 ii, tcs sp5 mp, tcs sp5 x, flim, smd. Basic training video for use of the leica confocal microscope at tufts. Conducting a sequential scan using the leica tcs sp5 confocal microscope. Leica application suite x las x is the one software platform for all leica microscopes. The leica tcs sp5 confocal fully covers a broad range of requirements in confocal and multiphoton imaging with excellent overall performance. This setup enables you to easily switch between conventional and resonant scanner. Technical specifications tcs sp5 aobs spectral confocal scanner mounted on a leica dm6000 upright fluorescent microscope.
Leica microsystems cms gmbh reserves the right to revise this. The information contained in this operating manual is subject to change without prior notice. The software described in this document is furnished under a license agreement which is included with the product. Leica sp5 general training manual sept 2010 robarts. Leica tcs sp5 lasaf new user guide asu core facilities.
The microscope is a leica dmi 6000 cs inverted microscope with a motorized xystage and zdrive motor focus on the microscope stand 100 nm step size as. Start the leica confocal software las available both as a desktop shortcut and the windows start menu. Confocal microscopy laboratory nus centre for bioimaging. En pdf, 2 mb tcs mp5flyer en pdf, 231 kb dedicated articles. This agreement specifies the permitted and prohibited uses of the product. Jan 04, 2018 this video shows how to configure the internal laser power wavelength etc. An introduction from your bosssupervisor is not enough the system has 5 detectors. Tcs stands for true confocal scanner and sp5 for the 5 spectrophotometer channels this system is equipped with. The leica lcs software can perform multidimensional image processing, fret, frap, and lambda scanning as well as controlling the motorized leica dm ire2 microscope. Sequential scanning using the leica tcs sp5 confocal laser scanning microscope this supplemental manual is the second section of a three part leica tcs sp5 user guide edited by donald pottle schepens 149 w dwp mar. Leica sp5 scanning confocal cdb equipment resource. With the combination of leica microsystems tcs sp5 confocal imaging instrumentation, single and multiple projects are easy to handle from single images that need to be evaluated at high standardization to 3d image stacks and 4d time series. If you select yes, the stage is going to move to all 4 corners to calibrate the position of the stage. The software will start the initialisation of the microscope stand and the scan head, and it cannot be used during this process.
The sp8 lightning confocal microscope allows you to make proper and detailed observations of fast biological processes. Review the manual to revert to an older back up of the software. Switch speed in an instant the new leica tcs sp5 ii comes with a tandemscanning system mounted on a sliding device. The leica tcs sp5 is made to meet your needs as a scientist who wants to reach higher. The microscope is a leica dmi 6000 cs inverted microscope with a motorized xystage and zdrive motor focus on the microscope stand 100 nm step size as well as a superz galvo stage 1500.
Consultation with the equipment manager is required before using this microscope. Rmbf leica sp5 aobs spectral confocal core facilities. Leica tcs sp5 ii the only broadband confocal technical documentation living up to life. Leica tcs sp5 confocal laser excitation lasers excitation wavelength common excitable fluorophores apeak absorbance empeak emission 405 laser diode 405 nm hoechst 33258 a350 em450 acma aminochloromethoxyacridine a430 em474 dapi 4,6diamidino2phenylindole a372 em456 alexa fluor 405 a402 em421. Even smallest series of throughput are possible in manual process steps. Leica tcs sp5 confocal laser scanning microscope user guide. Leica tcs sp2 aobs spectral confocal scanner mounted on a leica dm ire2 inverted fluorescent microscope.
Open source free software for a leica tcs sp5 confocal. Leica sp5 inverted confocal microscope with aresonant scanner allowing highspeed confocal imaging and is well suited for livecell imaging and highresolution morphological imaging. Remove the dust cover from the microscope turn on the mercury lamp a if you need to see the fluorescence through the eye pieces. National chiao tung university, bio photonics lab, bypraveen kumar. Leica application and technology support group, life science division.
Leica application and technology support group, life science division exton, pa 19341, usa leica tcs sp5 lasaf new user guide. This book is both a tutorial and a reference manual for the leica tcs. Leica sp5c spectral confocal laser scanning microscope. Leica dm6000 cfs upright multiphoton microscope equipped with a tcs sp5 mp laser confocal with both galvonomic and resonance scanners and with spectral hyd detectors. Pcmicroscope scanner power laser power laser key las af ok. An acoustooptical beam splitter aobs is used to split excitation. Your experimental work will have the benefit of superresolution, highspeed imaging, and the capability to image multiple fluorescent markers simultaneously. The leica tcs sp2 is supplied with the latest version of the licensed leica confocal software. The leica tcs sp5x confocal microscope system with multiphoton laser mp consists of an inverted microscope dmi 6000 cs with a xy scanning stage, z motorized focus as well as a superz galvo stage, for higher speed and repeatability. The leica sp5 confocal system has eight visible laser lines provided by a multiline argon laser, a diodepumped solid state laser, and orange and red helium neon lasers.
Leica microsystems growth confocal is an imaging system for entrylevel budgets that is fully upgradable as your research demands grow. To always keep the information up to date, the description of software functions was intentionally omitted from these operating instructions. Leica tcs sp5 x with two photon excitation laser the leica tcs sp5x twophoton click for fullsize image. In addition, this operating manual provides a rough overview of the operating principle of laser scanning microscopes. Only people who have had an introduction from kees straatman are allowed to operate the system. The leica tcs sp5 is supplied with the latest version of the licensed leica application suite advanced fluorescence. Image window robarts research institute leica sp5 training manual september 2010. Starting the lcs software on the leica tcs sp5 confocal microscope.
The content is designed to support beginners, experienced practitioners and scientists alike in their everyday work and experiments. View and download leica tcs sp5 user manual online. Science lab, the knowledge portal of leica microsystems, offers scientific research and teaching material on the subjects of microscopy. Training manual leica tcs sp5 confocal microscope september 2010. Helene javot, sp5 confocal microscope user manual, updated by mamta srivastava 2 022012, page 1 15 pcic plant cell imaging center bti, room 104b confocal leica sp5 user manual startup procedure. To maintain information on the most current level, the description of software functions was intentionally omitted from these operating instructions. How to use leica confocal microscope sp5 external laser. To maintain information on the most current level, the description of software functions was intentionally omitted from this operating manual. Open source free software for a leica tcs sp5 confocal microscope. Leica tcs sp5 confocal user manual only people who have had an introduction from kees straatman are allowed to operate the system. Leica dm2500 upright fluorescent microscope manuals.
The leica tcs sp5 ii growth confocal system without compromise, the leica tcs sp5 ii confocal system is equipped with the latest technology, offering high sensitivity and superb resolution. Jan 11, 2008 basic training video for use of the leica confocal microscope at tufts. The center operates a leica tcs sp5 fs fixed stage, upright microscope. To learn about the leica confocal software, use the book as a. An acoustooptical beam splitter aobs is used to split excitation light and emission light instead of filter blocks. This confocal is reserved for primary and joint cdb faculty and their personnel. Introduction the ibif confocal microscope is made available on a feeforusehour basis to all users who have been. It integrates confocal, widefield, stereo, superresolution, and lightsheet instruments from leica microsystems. This video shows how to configure the internal laser power wavelength etc.
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